中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (39): 6298-6303.doi: 10.3969/j.issn.2095-4344.2014.39.012

• 细胞外基质材料 extracellular matrix materials • 上一篇    下一篇

纤维蛋白胶介导内皮祖细胞再生心肌梗死血管的可行性

阿迪拉•阿扎提，赵  龙，周欣荣，刘  芬，陈邦党，马依彤   

  1. 新疆医科大学第一附属医院心脏中心，新疆维吾尔自治区乌鲁木齐市  830054
  • 出版日期:2014-09-17 发布日期:2014-09-17
  • 通讯作者: 马依彤，教授，主任医师，博士生导师，新疆医科大学第一附属医院心脏中心，新疆维吾尔自治区乌鲁木齐市 830054
  • 作者简介:阿迪拉?阿扎提，女，1969年生，新疆维吾尔自治区乌鲁木齐市人，维吾尔族，2008年新疆医科大学毕业，博士，主任医师，副教授，主要从事心肌组织工程研究。
  • 基金资助:

    国家自然青年科学基金项目(81000089)

Myocardial revascularization after myocardial infarction using endothelial progenitor cells combined with fibrin gel

Adila Azhati, Zhao Long, Zhou Xin-rong, Liu Fen, Chen Bang-dang, Ma Yi-tong   

  1. Cardiac Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Online:2014-09-17 Published:2014-09-17
  • Contact: Ma Yi-tong, Professor, Chief physician, Doctoral supervisor, Cardiac Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Adila Azhati, M.D., Chief physician, Associate professor, Cardiac Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81000089

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被引次数

1

摘要：

背景：有研究显示纤维蛋白胶可促进成肌细胞移植物的保持和生存，减少梗死范围并在梗死区诱导新生血管化。
目的：了解内皮祖细胞经可降解材料纤维蛋白胶移植到大鼠梗死心肌后的血管再生情况。
方法：将27只SD大鼠随机均分为3组，非心肌梗死组9只、心肌梗死即刻移植组9只与心肌梗死1周移植组9只。每个大组又再分为两个亚组，即移植人脐带源内皮祖细胞-纤维蛋白胶复合物的实验组与移植纤维蛋白胶的对照组。移植后3，8周处死，通过显微镜、免疫组织化学和心脏超声观察其在梗死心肌的血管再生和心功能改善情况。
结果与结论：显微镜观察到，实验组大鼠心脏和胸部之间有一些疏松的结缔组织，而其与对照组之间无明显差异。组织和免疫学观察发现，各实验组和对照组的心脏结构不易区分且相对正常，未发现血管瘤、血管畸形和肿瘤等。血管测量结果显示实验组和对照组之间，以及各实验组之间均无差异，并且实验组和对照组之间心功能检查也没有统计学意义。此次研究内皮祖细胞结果没有阳性表现，将修改并提高细胞通过纤维蛋白基质传递的方法策略，确信细胞传递系统提供的有益性和有效性将会进一步得到证实。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 材料相容性, 纤维蛋白胶, 心肌梗死, 内皮祖细胞, 移植, 血管再生, 国家自然科学基金

Abstract:

BACKGROUND: Studies have shown that fibrin glue can promote the survival of myoblast grafts, reduce infarct size and induce neovascularization of infarct zone.
OBJECTIVE: To understand the condition of revascularization of infarcted heart muscle using endothelial progenitor cells combined with degradable fibrin glue materials.
METHODS: A total of 27 Sprague-Dawley rats were randomized into three groups, 9 rats in each group: non-myocardial infarction group, immediate transplantation group and 1-week post-infarction transplantation group. Then, these three groups were sub-grouped into two groups, respectively: endothelial progenitor cells+fibrin glue group (experimental group) and fibrin glue group (control group). At 3 and 8 weeks after transplantation, the rats were sacrificed in each group. The revascularization and function of infracted heart muscle were observed by microscope, immunohistochemistry and echocardiography.
RESULTS AND CONCLUSION: Under the microscope, there were some lax connective tissues between the heart and chest in the experimental groups, but no difference existed between the experimental and control groups. The heart structure was normal relatively and difficult to be distinguished between the experimental and control groups histologically and immunologically, and there was no angeioma, vascular malformation and tumor. The number of revascularization of heart muscle showed no difference between experimental and control groups  as well as between different experimental groups. Additionally, there was no significant difference in cardiac function between experimental and control groups. Although there are no positive results of endothelial progenitor cells, we will modify and improve the strategy and believe that the cell delivery system is of benefit and efficacy.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: myocardial infarction, fibrin, endothelial cells

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